Technical Data
L2623-12A
LOXL2 (Lysyl Oxidase-like Protein 2, Lysyl Oxidase Related Protein 2, LOR2, Lysyl Oxidase Homolog 2, Lysyl Oxidase Related Protein WS9-14)
Description:
Lysyl Oxidase (LO) is an extracellular, copper-dependent enzyme that initiates the cross-linking of collagens and elastin by catalyzing the oxidative deamination of peptidyl lysine to alpha-aminoadipic-delta-semialdehyde. Members of LO family have diverse functions which include tumor suppression and cell adhesion and senescence. LOXL2, a 87kD protein, is a novel member of this family. It contains 3 potential N-linked glycosylation sites, 4 scavenger receptor cysteine-rich (SRCR) domains and highly conserved copper-binding and Lysyl-tyrosylquinone cofactor sites. It is a component of extracellular matrix that might be involved in cell adhesion and senescence. Role of LOXL2 has been implicated in malignant transformation.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa61-73 (AGQKRKHSEGRVE) of human LOXL2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human LOXL2. Species Crossreactivity: chimpanzee, canine, bovine. Species sequence homology: mouse, rat, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Saito,H., Papaconstantinou,J., Sato,H. and Goldstein,S. Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence. J. Biol. Chem. 272 (13), 8157-8160 (1997). 2. Jourdan-Le Saux,C., Tronecker,H., Bogic,L., Bryant-Greenwood,G.D., Boyd,C.D. and Csiszar,K. The LOXL2 gene encodes a new lysyl oxidase-like protein and is expressed at high levels in reproductive tissue. J. Biol. Chem. 274 (18), 12939-12944 (1999). 3. Molnar,J., Fong,K.S., He,Q.P., Hayashi,K., Kim,Y., Fong,S.F., Fogelgren,B., Szauter,K.M., Mink,M. and Csiszar,K. Structural and functional diversity of lysyl oxidase and the LOX-like proteins. Biochim. Biophys. Acta 1647 (1-2), 220-224 (2003).