Technical Data
Media
Listeria Enrichment Broth (Powder)
L2650-05
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone17.0
Dextrose2.5
Yeast Extract6.0
Pancreatic Digest of Soybean Meal3.0
Sodium Chloride5.0
Disodium Phosphate2.5
Cyclohexamide0.5
Naldixic Acid0.04
Acriflavine0.015
Total36.55/liter
Selective enrichment of Listeria monocytogenes from milk.


Directions per Liter: Dissolve 36.5g in 800-900ml of ddH2O. Heat with repeated stirring to dissolve completely. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Aliquot into sterile containers. Autoclave at 121C for 15 minutes. Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder

Solubility: Gold to orange-amber with green opalescent top; may have slight precipitate.

pH: 7.3 0.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.