		Technical Data
L4500-06A	LRP, MVP (Lung-resistance Related Protein, Major Vault Protein, 2310009M24Rik, VAULT1)

Description:
MVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP.

Cellular Localization: Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:50-1:100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8L602	Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

This antibody reacts with a 104 to 110kD protein known as Major Vault Protein (MVP). Species Crossreactivity: Crossreacts with Human and Rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Abbondanza C et al. Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell. J Cell Biol 141:1301-10 (1998).