Luciferin D-, Firefly, Potassium Salt (4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid Potassium, D-Luciferin)
|Biochemicals||Storage: -20°CShipping: Blue Ice|
Luciferin is the substrate for luciferase and is used in highly sensitive bioluminescence assays for the detection of ATP; the generation of light is directly proportional to the concentration of ATP. Also used in detection of the luciferase marker gene.
Methanol, acetone, ethyl acetate, DMF. Slightly soluble in water.
E385±0.2nm (0.5M Na2CO3, pH 11.5):
Specific Gravity (H20 = 1.0):
Water Content (KF):
Storage and Stability:
Store lyophilized powder at -20°C, dessicated and protected from light . Reconstitute with ddH2O and bring solution to a pH of 6.0-6.3. Reconstituted product can be stored protected from light at °4C for up to 2 weeks. or at -20°C for several months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: At a pH greater than 6.3, D-Luciferin may undergo a slow racemization from the D- to the L- isomer.
Purity: ~99% (HPLC)
Form: Supplied as a pale yellow to yellow, crystalline powder
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: 1. Belleannée, C. et al., (2012) PLoS ONE 7(4): e34996. doi:10.1371/journal.pone.0034996. 2. Rodriguez, D.A. et al., (2009) Mol. Biol. Cell 20:2297-2310.