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Technical Data |
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L6007 |
Luciferin D-, Firefly, Potassium Salt (4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid Potassium, D-Luciferin) |
50mg 100mg |
| Biochemicals | Storage: -20°CShipping: Blue Ice |
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Luciferin is the substrate for luciferase and is used in highly sensitive bioluminescence assays for the detection of ATP; the generation of light is directly proportional to the concentration of ATP. Also used in detection of the luciferase marker gene. Solubility: Methanol, acetone, ethyl acetate, DMF. Slightly soluble in water. UV Absorpbance E385±0.2nm (0.5M Na2CO3, pH 11.5): 17,500 Specific Gravity (H20 = 1.0): 1.425 Water Content (KF): 9% Impurities: 0.10% Melting Point: >200°C (>391°F) Boiling Point: >300°C (>571°F) Storage and Stability: Store lyophilized powder at -20°C, dessicated and protected from light . Reconstitute with ddH2O and bring solution to a pH of 6.0-6.3. Reconstituted product can be stored protected from light at °4C for up to 2 weeks. or at -20°C for several months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: At a pH greater than 6.3, D-Luciferin may undergo a slow racemization from the D- to the L- isomer. CAS Number: 115144-35-9 Molecular Formula: C11H7N2O3S2K Molecular Weight: 318.4 |
Source: Firefly Purity: ~99% (HPLC) Form: Supplied as a pale yellow to yellow, crystalline powder Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application reference: 1. Belleannée, C. et al., (2012) PLoS ONE 7(4): e34996. doi:10.1371/journal.pone.0034996. 2. Rodriguez, D.A. et al., (2009) Mol. Biol. Cell 20:2297-2310.
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