Technical Data
L9190-08
Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA)
Description:
CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles (1). It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion (2). CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice (3). LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases (4).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoaffinity chromatography, Immunoprecipitation, Western Blot and Immunoelectron microscopy. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k1D4BPurified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Plasma membrane fraction of mouse embryo NIH3T3 cell line
Purity:
Purified
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0.
Specificity:
Recognizes mouse CD107a/LAMP-1 (Mr 110-140kD with a core protein of Mr ~40kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen, J.W., W. Pan, M.P. d’Souza and J.T. August. 1985. Arch. Biochem. Biophys. 239:574. 2. Arterburn, L.M., B.J. Earles, and J.T. August. 1990. J. Biol. Chem. 265:7419. 3. Chen, J.W., G.L. Chen, M.P. d’Souza, T.L. Murphy, and J.T. August. 1986. Biochem. Soc. Symp. 51:97. 4. Fukuda, M. 1991. J. Biol. Chem. 266:21327. 5. Southern Biotechnology Associates, Inc. Unpublished observations. 6. Rohrer, J., A. Schweizer, D. Russell, and S. Kornfeld. 1996. J. Cell. Biol. 132:565.