Technical Data
L9200-07
Lysozyme (Muramidase)
Description:
Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of b-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme "g". Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacterial susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and chemically is distinct from the egg white enzyme.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:20 using frozen and paraffin-embedded tissue and cytological material.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F244Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Human lysozyme
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human lysozyme. Does not react with rabbit. Blocks the catalytic activity of human lysozyme. For immunohistochemical detection of histiocytic neoplasia and myeloid leukemias. Monocytic leukemia cells show strong positive immune reaction. Weak reaction is also found with granulocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.