		Technical Data
M1011-50D	M13, fd, F1 Bacteriophages, Filamentous (FITC)

Description:
Applications:
Suitable for use in ELISA and Phage Display
(immunoassays for the identification of recombinant antigen- or antibody-phages; detection limit: 10e7 phage particles). Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml. The detection limit for M13, fd, F1 Bacteriophages, Filamentous is 10e7 phage particles.
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG2b	5F167	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	E. coli	Mouse

Concentration:

~0.4mg/ml

Immunogen:

fd phages from E. coli F+ strain (JM109).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in Tris buffer, pH 8.0, 0.5% BSA, 0.09% sodium azide. Labeled with FITC.

Specificity:

Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171,103-109 (1994). 2. Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999). 3. Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotechnology 19, 75-78 (2001).