Technical Data
M13, fd, F1 Bacteriophages, Filamentous (FITC)
Suitable for use in ELISA and Phage Display
(immunoassays for the identification of recombinant antigen- or antibody-phages; detection limit: 10e7 phage particles). Other applications not tested.

Recommended Dilutions:
ELISA: 1ug/ml. The detection limit for M13, fd, F1 Bacteriophages, Filamentous is 10e7 phage particles.
Optimal dilution to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2b5F167Affinity Purified
50ul-20°CBlue IceE. coliMouse
fd phages from E. coli F+ strain (JM109).
Purified by Protein A affinity chromatography.
Supplied as a liquid in Tris buffer, pH 8.0, 0.5% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Micheel B, Heymann S, Scharte G, Böttger V, Vogel V, Dübel S, Breitling F, Little M, Behrsing O. Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages. J. Immunol. Methods 171,103-109 (1994). 2. Kneissel S, Queitsch I, Petersen G, Behrsing O, Micheel B, Dübel S. Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae). J. Mol. Biol. 288, 21-28 (1999). 3. Rondot S, Koch J, Breitling F, Dübel S: A helper phage to improve single-chain antibody presentation in phage display. Nature Biotechnology 19, 75-78 (2001).