Technical Data
Media
M63 Medium (Powder)
M1015
Molecular Biology Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Ammonium Sulfate2.0
Potassium Phosphate mono13.6
Ferrous Sulfate0.0005
Total15.6g/L
Not included:
Carbohydrate Solution:
Carbohydrate(glucose, gycerol)20g/100ml
Add carbohydrate to dH2O. QS to 100ml. Filter sterilize.
Magnesium Sulfate Solution (MgSO4•7H2):
MgSO4•7H2O24.65g/100ml
Add MgSO4•7H2O to dH2O QS to 100ml. Filter sterilize.
Buffered salt formulation used as a base to prepare minimal media for propagation of E. coli and plasmid amplification. A complete media that requires one or more additional salts and carbon source.

Appearance:
White, homogeneous, free flowing powder

Solubility:
Colorless, clear, complete

pH:
4.3 ± 0.4


Directions per Liter Dissolve 15.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application references: 1. Martinez-Medina, M. et al., (2009) BMC Microbiology, 9:202. 2. Naves, P. et al., (2010) J. Hospital Infection 76; 165-170.