Technical Data
Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim, Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
Molecular Biology Storage: -20CShipping: RT
Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, macrophages and endothelial cells. This colonly stimulating factor is involved in both the development and function of monocytes/macropahges. Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of 158 amino acid residues.

Biological Activity:
Human M-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is 5 ng/ml, corresponding to a specific activity of 2x10e5 units/mg.
Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC analyses). Endotoxin: 0.1ng/ug (1EU/ug).
Form: Supplied as a lyophilized powder from a sterile filtered solution in PBS, pH 8.0..Reconstitute with sterile, ddH2O to 0.1-0.5 mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.