Technical Data
M1201-03A
Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)
2ug
10ug
10ug
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Technical Data |
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M1201-03A |
Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo Description: Recombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4kD. Recombinant M-CSF is purified by proprietary chromatographic techniques. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel. Biological Activity: Mouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg. Endotoxin: 0.1ng/ug (IEU/ug) of Recombinant Murine M-CSF. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard. Solubility: Reconstitute the lyophilized mouse M-CSF in sterile dH2O to 100ug/ml, which can then be further diluted with other aqueous solutions. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 36.4kD |
Source: E. coli Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Concentration: ~1mg/ml Form: Supplied as a lyophilized powder in 5mM Tris, pH 8.0, 100mM sodium chloride. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Horm. Metab. Res. 38(3): 193-196 (2006). 2. J. Am. Coll. Cardiol. 47(3): 626-634 (2006). 3. Am. J. Reprod. Immunol. 55(3): 226-231 (2006). 4. J. Int. Med. Res. 33(6): 654-660 (2005). 5. Arthritis Rheum. 52(12): 3819-3828 (2005). 6. J. Interferon Cytokine Res. 25(11): 674-683 (2005).
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