Technical Data
Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)
Molecular Biology Storage: -20CShipping: Blue Ice
Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo

Recombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4kD. Recombinant M-CSF is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser.

Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel.

Biological Activity:
Mouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.

0.1ng/ug (IEU/ug) of Recombinant Murine M-CSF.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard.

Reconstitute the lyophilized mouse M-CSF in sterile dH2O to 100ug/ml, which can then be further diluted with other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder in 5mM Tris, pH 8.0, 100mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Horm. Metab. Res. 38(3): 193-196 (2006). 2. J. Am. Coll. Cardiol. 47(3): 626-634 (2006). 3. Am. J. Reprod. Immunol. 55(3): 226-231 (2006). 4. J. Int. Med. Res. 33(6): 654-660 (2005). 5. Arthritis Rheum. 52(12): 3819-3828 (2005). 6. J. Interferon Cytokine Res. 25(11): 674-683 (2005).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.