Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo
Recombinant Mouse M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4kD. Recombinant M-CSF is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser.
Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel.
Mouse M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.
0.1ng/ug (IEU/ug) of Recombinant Murine M-CSF.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of M-CSF as a Reference Standard.
Reconstitute the lyophilized mouse M-CSF in sterile dH2O to 100ug/ml, which can then be further diluted with other aqueous solutions.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a lyophilized powder in 5mM Tris, pH 8.0, 100mM sodium chloride.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.