Technical Data
Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim, Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
Growth Factors, Cytokines Storage: -20CShipping: RT
Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo.

Recombinant Human M-CSF is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8kD.

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.

Biological Activity:
M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found <1ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg.

Less than 0.1ng/ug (IEU/ug) of M-CSF.

Storage and Stability:
Store powder dessicated at -20C. Liquid can be stored at 4C for 2-7 days, long term at -20C. For long term it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to prevent repeated freeze-thaw cycles.

Source: Recombinant, Human (E. coli)
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
Form: Supplied as a lyophilized powder in HSA, Manntiol. Reconstitute sterile ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. SHIP2 is recruited to the cell membrane upon macrophage colony-stimulating factor (M-CSF) stimulation and regulates M-CSF-induced signaling. Wang Y, Keogh RJ, Mitchell CA, Schurmans S, J Immunol 2004 Dec 1;173(11):6820-30 2. Serum levels of matrix metalloproteinase 3 and macrophage colony-stimulating factor 1 correlate with disease activity in ankylosing spondylitis. Yang C, Gu J, Baeten D, Maksymowych WP, Arthritis Rheum 2004 Oct 15;51(5):691-9 3. Synergistic effect of interleukin (IL)-1alpha and ceramide analogue on the production of IL-6, IL-8, and macrophage colony-stimulating factor by endometrial stromal cells. Kawano Y, Fukuda J, Matsumoto H, Fertil Steril 2004 Oct;82 Suppl 3:1043-7 4. Membrane-bound macrophage colony-stimulating factor mediated auto-juxtacrine downregulates matrix metalloproteinase-9 release on J6-1 leukemic cell. Rao Q, Zheng GG, Lin YM, Exp Biol Med (Maywood) 2004 Oct;229(9):946-53 5. Preoperative sensitivity and specificity for early-stage ovarian cancer when combining cancer antigen CA-125II, CA 15-3, CA 72-4, and macrophage colony-stimulating factor using mixtures of multivariate normal distributions. Skates SJ, Horick N, Xu FJ, van der Zee AG, J Clin Oncol 2004 Oct 15;22(20):4059-66 6. Discrimination of heterozygous from wild-type mice bearing a mutation in the macrophage-colony-stimulating factor gene using capillary electrophoresis. Techel D, Bergmann C, Jost MM, Lab Anim (NY) 2004 Sep;33(8):33-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.