Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Human (MIP-1a, CCL3)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to human MIP-1a expressed in E. coli.

Molecular Weight:

Biological Activity:
ED50 range=1-5ng/ml, determined by the dose dependent chemotaxis of human THP-1 cells. Optimal concentration for individual application should be determined by a dose response assay.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Barratt-Boyes, S.M., M.I. Zimmer, L.A. Harshyne, E.M. Meyer, S.C. Watkins, S. Capuano, M. Murphey-Corb, L.D. Falo, and A.D. Donnenberg (2000) Maturation and trafficking of monocyte-derived dendritic cells in monkeys: Implications for dendritic cell-based vaccines. J. Immunol. 164:2487-2495 (used with dendritic cells from rhesus monkey). 2. Bluman, E.M., K.J. Bartynski, B.R. Avalos, and M.A. Caligiuri (1996) Human natural killer cells produce abundant macrophage inflammatory protein-1 alpha in response to monocyte-derived cytokines. J. Clin. Invest. 97(12):2722-2727. 3. Stellato, C., M.E. Brummet, J.R. Plitt, S. Shahabuddin, F.M. Baroody, M.C. Liu, P.D.Ponath, and L.A. Beck (2001) Cutting edge: Expression of the C-C chemokine receptor CCR3 in human airway epithelial cells. J. Immunol. 166 (3):1457-1461. 4. Vaddi, K., M. Keller and R. Newton (1990) The Chemokine Factsbook pp. 107-117. Academic Press, Inc. San Diego, CA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.