Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Mouse (MIP-1 alpha, CCL3)
Molecular Biology Storage: -20CShipping: RT
Recombinant murine Macrophage Inflammatory Protein-1 alpha is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7.8kD.
Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as Interleukin 1 (IL-1), IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.

Amino Acid Sequence:
First five N-terminal amino acids was determined and was found to be Ala-Pro-Tyr-Gly-Ala.

Biological Activity:
Murine Macrophage Inflammatory Protein-1 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of BALB3/C splenocytes using 1-10ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Recombinant, murine produced in E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Fujita, H., et al., J. Invest. Dermatol. 122(5): 1331-1333 (2004). 2. Herrlinger, U., et al., J. Neurooncol. 66(1-2): 147-154 (2004). 3. Yoshida, S., et al., J. Leukoc. Biol. 73(1): 137-144 (2003). 4. Ousman, S.S. & David, S., J. Neurosci. 21(13): 4649-4656 (2001).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.