Technical Data
Macrophage Inflammatory Protein 1 beta, Recombinant, Human (MIP-1b, CCL4)
Molecular Biology Storage: -20CShipping: RT
Recombinant Human MIP-1 is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton.

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
rHuMIP-1 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml.

0.1ng/ug (IEU/ug) of rHuMIP-1.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of MIP-1 as a Reference Standard.

Reconstitute the lyophilized rHuMIP-1 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Glycosaminoglycan disaccharide alters the dimer dissociation constant of the chemokine MIP-1 beta. McCornack MA, Boren DM, Biochemistry 2004 Aug 10;43(31):10090-101 2. [Serum and cerebrospinal fluid concentration of inflammatory proteins MIP-1-alpha and MIP-1-beta and of interleukin 8 in the course of borreliosis] Grygorczuk S, Pancewicz S, Swierzbinska R, Neurol Neurochir Pol 2003 Jan-Feb;37(1):73-87 3. Levels of macrophage inflammatory protein 1 alpha (MIP-1 alpha) and MIP-1 beta in intervillous blood plasma samples from women with placental malaria and human immunodeficiency virus infection. Chaisavaneeyakorn S, Moore JM, Othoro C, Nahlen BL, Clin Diagn Lab Immunol 2003 Jul;10(4):631-6 4. Human neuronal cells (NT2-N) express functional substance P and neurokinin-1 receptor coupled to MIP-1 beta expression. Li Y, Douglas SD, Lai J, Ho W, J Neurosci Res 2003 Feb 15;71(4):559-66 5. Characterization of the role of the N-loop of MIP-1 beta in CCR5 binding. Bondue A, Jao SC, Parmentier M, Biochemistry 2002 Nov 19;41(46):13548-55 6. [Concentration of macrophage inflammatory proteins MIP-1 alpha and MIP-1 beta and interleukin 8 in erythema migrants] Grygorczuk SS, Pancewicz S, Zajkowska J, Pol Merkuriusz Lek 2002 Jun;12(72):458-61.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.