Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Recombinant Mouse MIP-3beta produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. Recombinant MIP-3b is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala.
Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Recombinant MIP-3 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration of 10-100 ng/ml.
0.1ng/ug (IEU/ug) of MIP-3b.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-3beta as a Reference Standard.
It is recommended to reconstitute the lyophilized Mouse MIP-3 b in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution murine MIP-3b should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a lyophilized powder without additives.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.