Macrophage Inflammatory Protein 3 beta, Recombinant, Mouse (MIP-3b, Exodus-3, ELC)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
Recombinant Mouse MIP-3beta produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. Recombinant MIP-3b is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala.
Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Recombinant MIP-3 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration of 10-100 ng/ml.
0.1ng/ug (IEU/ug) of MIP-3b.
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-3beta as a Reference Standard.
It is recommended to reconstitute the lyophilized Mouse MIP-3 b in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized MIP-3beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution murine MIP-3b should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source: E. coli
Purity: 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form: Supplied as a lyophilized powder without additives.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dendritic cells express CCR7 and migrate in response to CCL19 (MIP-3beta) after exposure to Helicobacter pylori.
Microbes Infect 2006 Mar;8(3):841-50
2. Inhibition of generation of cytotoxic T lymphocyte activity by a CCL19/macrophage inflammatory protein (MIP)-3beta antagonist.
J Biol Chem 2004 Sep 24;279(39):40276-82
3. Production of macrophage inflammatory protein 3alpha (MIP-3alpha) (CCL20) and MIP-3beta (CCL19) by human peripheral blood neutrophils in response to microbial pathogens.
Infect Immun 2003 Jan;71(1):524-6
4. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR.
Eur J Immunol 2002 May;32(5):1230-41
5. Expression of CCR-7, MIP-3beta, and Th-1 chemokines in type I IFN-induced monocyte-derived dendritic cells: importance for the rapid acquisition of potent migratory and functional activities.
Blood 2001 Nov 15;98(10):3022-9
6. Neutrophils produce biologically active macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19.
Eur J Immunol 2001 Jul;31(7):1981-8