Technical Data
M1499-15C
MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A, MAD2-like 1, MAD2-like Protein 1, Homolog of MAD2 (S. cerevisiae), HsMAD2, Human Mitotic Feedback Control Protein Madp2, MAD2, MAD2 Mitotic Arrest Deficient-like 1, MAD2A, MD2L1, Mitotic Arrest De
Description:
The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide which maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085).
Purity:
Purified by immunoaffinity chromatography.
Form
As reported
Specificity:
Specific for human MAD2 protein. Other species not tested. Reactivity with mouse MAD2 is unlikely.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wu, G., Glickstein, S., Liu, W., Fujita, T., Li, W., Yang, Q., Duvoisin, R., and Wan, Y. (2007) The Anaphase-promoting Complex Coordinates Initiation of Lens Differentiation, 18, 1018-1029.