		Technical Data
M2137	Malonaldehyde Deoxyguanosine Adduct (M1G-R)

Description:
M2137 has been used for immunoaffinity isolation of M1G prior to GC/MS. Less than 5 fmol of M1G-R in MDA-modified DNA can be detected by an immunoslot blot assay for the quantitation of MDA-modified DNA using this antibody.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:20,000
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	4i71	Affinity Purified

Size		Storage	Shipping	Source	Host
10ug		-20°C	Blue Ice		Mouse

Concentration:

~0.4mg/ml

Immunogen:

Malonaldehyde-deoxy guanosine adduct, 3-beta-erythro-pentofuranosylpyrimido[1,2-alpha]purin-1093H0-one (abbreviated M1G-R)

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 50% glycerol.

Specificity:

Recognizes M1G-R.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Leuratti, C., Singh, R., Lagneau, C., Farmer, P.B., Plastaras, J.P., Marnett, L.J., Shuker, D.E., Carcinogenesis, 19 : 101-106 (1998)2. Fink, S.P., Reddy, G.R., Marnett, L.J., Proc. Natl Acad Sci., 94 : 8652-8657 (1997) 3. Rouzer, C.A., et. al., Chem Res. Tox., 10 : 181-188 (1997) 4. Niedernhofer, L.J., et. al., Chem Res. Tox., 10 : 556-561 (1997) 5. Sevilla, C.L., et. al., Chem Res. Tox., 10 : 172-180 (1997)