Technical Data
M2155-18
Maltose Phosphorylase (Biotin)
Description:
In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reaction
maltose + phosphate D-glucose + beta-D-glucose 1-phosphate
Thus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism.

Applications:
Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000-1:20,000; 1ug of Maltose Phosphorylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per Goat IgG molecule

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceGoat
Concentration:
~10mg/ml
Immunogen:
Maltose Phosphorylase (E. coli)
Purity:
Purified by delipidation, salt fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
Specificity:
Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Maltose Phosphorylase (E. coli).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.