Technical Data
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (ERK1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase. While multiple ERK1/2 MAP3Ks have been identified, including the Raf family, Mos, and Tpl2/Cot, MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate ERK1/p44 and ERK2/p42 through phosphorylation of activation loop residues Thr202/ Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). ERK1/2 are negatively regulated by a family of dual-specificity (Thr/ Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Flow Cytometry: 1:100
Western Blot: 1:2000
Immunohistochemistry: 1:200
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul-20°CBlue IceHumanRabbit
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
As reported
Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Recognizes endogenous levels of p42 and p44 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not crossreact with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. Species crossreactivity: mouse, rat, monkey, pig, hamster, bovine, dog, drosophila, c. elegans, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320–44. (2) Baccarini, M. (2005) FEBS Lett 579, 3271–7. (3) Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227–39. (4) Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291–310. (5) Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151–74. (6) Murphy, L.O. and Blenis, J. 2006) Trends Biochem Sci 31, 268–75. (7) Dalby, K.N. et al. (1998) J Biol Chem 273, 1496–505. (8) Marais, R. et al. (1993) Cell 73, 381–93. (9) Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815–24. (10) Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203–13.