Technical Data
M2352-20N
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Description:
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in
response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have
been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG11C1045Purified
SizeStorageShippingSourceHost
100ul4°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, less than 0.1% sodium azide, 2mg/ml BSA. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes p44/42 MAPK, phosphorylated at (Thr202/Thr204). Species Crossreactivity: mouse, rat, hamster, monkey, mink, chicken, D. melanogaster, xenopus, zebrafish, bovine, canine, poricine, S. cerevisiae, Ce C. elegans, horse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. (2) Baccarini, M. (2005) FEBS Lett 579, 3271-7. (3) Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. (4) Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. (5) Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31,
151-74. (6) Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. (7) Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. (8) Marais, R. et al. (1993) Cell 73, 381-93. (9) Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. (10) Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.