		Technical Data
M2410	MART-1 (Melanoma Antigen Recognized by T-cells 1, Melan-A)

Description:
MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein A.
Western Blot: 0.5-1ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. M2410-12 is preferred for this application.
Optimal dilutions to be determined by the researcher.

Positive Control:
CaC1 melanoma cells and melanomas

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2b,k	0.N.396	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Recombinant human MART-1 protein. Cellular Localization: Cytoplasmic

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2410X.

Specificity:

Recognizes human MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial or mesenchymal origin. Species Crossreactivity: Equine. Does not crossreact with mouse or rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

US Biological Application References: 1. Postovit, L.-M. et al., Stem Cells 24: 501-505 (2005). 2. Kulesa, P.M., PNAS 103: 3752-3757 (2006). General References: 1. Milani, V., et al., Int. Immunol. 17(3): 257-268 (2005). 2. Maliver, P., et al., J. Vet. Med. A. Physiol. Pathol. Clin. Med. 51(9-10): 413-415 (2004). 3. Kucher, C., et al., Am. J. Dermatopathol. 26(6): 452-457 (2004). 4. Chapatte, L., et al., J. Immunol. 173(10): 6033-6040 (2004). 5. Tiwari, R., et al., Peptides 25(11): 1865-1871 (2004). 6. Shabrawi-Caelen, L.E., et al., Am. J. Dermatopathol. 26(5): 364-366 (2004). 7. Kawakami, Y., et al., J. Immunol. Methods 202(1): 13-25 (1997). 8. Marincola, F.M., et al., J. Immunother. Emphasis Tumor Immunol. 19(3): 192-205 (1996).