Technical Data
M2425-31
Matrix Metalloproteinase 9 (MMP-9, Collagenase 4, Gelatinase B)
Description:
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000 detected MMP-9 on 10ug of L6 lysates at ~98kD.
Immunohistochemistry: 1:100 in frozen acetone fixed tissues.
Immunoprecipitation: 1:500
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Positive Control:
HL60 cell lysate, U937 cell lysate lung

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli expressed active rat 92kD type IV collagenase (catalytic domain) (Accession# NP_004985).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat Matrix Metalloproteinase 9 pro and active forms. Exhibits no crossreactivity with other MMP family members. Species Crossreactivity: mouse and human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Greenwald, R.A., Ann. NY Acad. Sci. 732: 273-179 (1994). 2. Van wart, H.E., et al., Proc. Natl. Acad. Sci. USA. 87: 5578-5582 (1990). 3. Goldberg, G.I., et al., J. Biol. Chem. 267: 4583-4591 (1992). 4. Christopolous, T.A., et al., Inter. J. Oncology 24: 1-5 (2004).