Technical Data
M2441-01
Matrix Metalloproteinase MT1 (MT1 MMP, Membrane Type 1, MMP-14)
Description:
Matrix metalloproteinases (MMPs) are Zn 2+ and Ca 2+-dependent endopeptidases which function in the turnover of extracellular matrix components. Presently, eighteen secreted MMPs and five membrane-type MMPs (25) are known to be expressed in vertebrates. Human MT1-MMP consists of 559 amino acid residues with a calculated Mr of 63516.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 8-20ug/ml
Immunohistochemistry (Paraffin): 20ug/ml. Requires periodate-lysine-paraformaldehyde for 18-24 hours at 4C.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q527Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding to aa160-173, REVPYAYIREGHEK of human membrane-type 1 matrix metalloproteinase.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free).
Specificity:
Recognizes human MT1-MMP. For unknown reasons this antibody does not react on Westerns to Cat# M2429-06. Does not crossreact with mouse, rat or porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sato, H., et al., Nature 370: 61-65 (1994). 2. Ueno, H., et al., Cancer Res. 57: 2055-2060 (1997). 3. Namura, H., et al., Cancer Res. 55: 3263-3266 (1995). 4. Nakamura, H., et al., Cancer Res. 59: 467-473 (1999).