		Technical Data
M2441-01	Matrix Metalloproteinase MT1 (MT1 MMP, Membrane Type 1, MMP-14)

Description:
Matrix metalloproteinases (MMPs) are Zn 2+ and Ca 2+-dependent endopeptidases which function in the turnover of extracellular matrix components. Presently, eighteen secreted MMPs and five membrane-type MMPs (2–5) are known to be expressed in vertebrates. Human MT1-MMP consists of 559 amino acid residues with a calculated Mr of 63516.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 8-20ug/ml
Immunohistochemistry (Paraffin): 20ug/ml. Requires periodate-lysine-paraformaldehyde for 18-24 hours at 4ºC.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	2Q527	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Peptide corresponding to aa160-173, REVPYAYIREGHEK of human membrane-type 1 matrix metalloproteinase.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free).

Specificity:

Recognizes human MT1-MMP. For unknown reasons this antibody does not react on Westerns to Cat# M2429-06. Does not crossreact with mouse, rat or porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Sato, H., et al., Nature 370: 61-65 (1994). 2. Ueno, H., et al., Cancer Res. 57: 2055-2060 (1997). 3. Namura, H., et al., Cancer Res. 55: 3263-3266 (1995). 4. Nakamura, H., et al., Cancer Res. 59: 467-473 (1999).