		Technical Data
M2690-19B	MCAF (MBD1-containing Chromatin Associated Factor, MCAF1, Activating Transcription Factor 7 Interacting Protein 1, ATF7IP, ATF Interacting Protein, ATF-IP, ATFa-associated Modulator, hAM, p621)

Description:
MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:5000-1:20,000
Immunohistochemistry: Frozen and paraffin sections
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa Nuclear Extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa25-100 of human MCAF (AAQ92978.1)(GeneID 55729).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.1% sodium azide

Specificity:

Recognizes human MCAF. Species Sequence Homology: mouse based upon conservation between this ortholog and human MCAF at the site to which the epitope maps.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Fujita N, Watanabe S, Ichimura T, Ohkuma Y, Chiba T, Saya H, Nakao M. MCAF Mediates MBD1-Dependent Transcriptional Repression. Mol Cell Biol. 2003 Apr 15; 23(8): 2834-2843.