Technical Data
MCP3, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 3)
Molecular Biology Storage: -20CShipping: RT
Human macrophage/monocyte chemotactic protein-3 (MCP-3) is a 8.5kD protein containing 76 amino acids residues. It plays an important role in the inflammatory response of blood monocytes, tissue macrophages, and eosinophils.

The gene encoding this protein encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.

Source: E. coli

Form: Lyophilized protein with no additives.

Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0C.

Purity: Greater than 99% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng perug of MCP-3.

Reconstitution: The lyophilized MCP-3 is soluble in water and most aqueous buffers. The lyophilized MCP-3 can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored frozen for future use.

Biological Activity: Human MCP-3 produced a 10-fold increase in chemotaxis at 100 ng/ml compared to the medium control in an assay using differentiated HL-60 cells plated on a primary endothelial cell layer. The assays were done using microporous cell culture inserts of 3 pore size that were not protein coated.

Responding Cells (partial list): Monocytes, basophils, eosinophils

Concentration Range for most in vitro applications: 5.0-50 ng/ml

Molecular Weight:
Source: E. coli

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.