Technical Data
MCP4, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 4)
Molecular Biology Storage: -20CShipping: RT
Recombinant Human MCP-4 is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Leu.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
MCP-4 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-4 to chemoattaract human monocytes at 10-100ng/ml.

0.1ng/ug (IEU/ug) of Human Monocyte Chemotactic Protein-4.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-4 as a Reference Standard.

Reconstitute the lyophilized MCP-4 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Monocyte chemotactic protein-4 (MCP-4; CCL-13): a biomarker of asthma. Kalayci O, Sonna LA, Camargo CA Jr, J Asthma 2004 Feb;41(1):27-33 2. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Meyer-Hoffert U, Lezcano-Meza D, Montes-Vizuet AR, Int Arch Allergy Immunol 2003 Aug;131(4):264-71 3. IL-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells. Nakamura H, Luster AD, Jedrzkiewicz S, Yamaguchi K, Am J Physiol Lung Cell Mol Physiol 2001 Nov;281(5):L1288-302 4. Potential role for monocyte chemotactic protein-4 (MCP-4) in monocyte/macrophage recruitment in acute renal inflammation. Chakravorty SJ, Howie AJ, Gentle D, J Pathol 2001 Jun;194(2):239-46 5. Cloning of equine chemokines eotaxin, monocyte chemoattractant protein (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4 in dermal fibroblasts. Benarafa C, Cunningham FM, Horohov DW, Vet Immunol Immunopathol 2000 Oct 31;76(3-4):283-98 6. Monocyte chemoattractant protein (MCP)-4 expression in the airways of patients with asthma. Induction in epithelial cells and mononuclear cells by proinflammatory cytokines. Lamkhioued B, Garcia-Zepeda EA, Nakamura H, Allakhverdi Z, Am J Respir Crit Care Med 2000 Aug;162(2 Pt 1):723-32.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.