Technical Data
M2825-12
MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)
Description:
Application(s):
Flow Cytometry (cell permeabilization required)
Immunocytochemistry (cytospin preparations)
Immunohistochemistry (frozen sections, weak in paraffin sections)
Western Blot
Suggested starting dilution is 1:20. Optimal conditions must be determined individually for each application.
TypeIsotypeCloneGrade
MabIgG26D555Supernatant
SizeStorageShippingSourceHost
125ug-20°CBlue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
Recombinant human MRP1 (multidrug resistance-associated protein 1) (aa 986-1204).
Purity:
Supernatant
Form
Liquid. Antibody in serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
Specificity:
Species Crossreactivity: HuRecognizes an internal epitope of human MRP1, overexpressed in various human non-P-glycoprotein MDR tumor cell lines. Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line: S.P. Cole, et al.; Science 258, 1650 (1992)
2. Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies: M.J. Flens, et al.; Cancer Res. 54, 4557 (1994)
3. The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump: G.J.R. Zaman, et al.; PNAS 91, 8822 (1994)
4. Membrane transport proteins associated with drug resistance expressed in human melanoma: D. Schadendorf, et al.; Am. J. Pathol. 147, 1545 (1995)
5. Tissue distribution of the multidrug resistance protein: M.J. Flens, et al.; Am. J. Pathol. 148, 1237 (1996)
6. Expression of the multidrug resistance-associated protein (MRP) gene in primary non-small-cell lung cancer: K. Nooter, et al.; Ann. Oncol. 7, 75 (1996)
7. Optimal immunocytochemical and flow cytometric detection of P-gp, MRP and LRP in childhood acute lymphoblastic leukemia: M.L. Den Boer, et al.; Leukemia 11, 1078 (1997)
8. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000)