Technical Data
M2825-12M
MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)
Description:
MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors .

Applications:
Suitable for use in Immunocytochemistry, Flow Cytometry, Immunoprecipitation and Transport inhibition. Not recommended for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: use on paraformaldehyde (0.5% in PBS) fixed cytospin preparations
Flow Cytometry: 0.2-1ug/ml after fixing cells in 0.5% paraformaldehyde, followed by suitable fluorescent tagged anti-mouse reagent (6)
Transport Inhibition: Inhibits the ATP-dependent transport activity of MRP1 in inside-out membrane vessicles.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6D559Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
~0.04mg/ml
Immunogen:
Non-denatured membranes prepared from the human small cell lung cancer cell line H69AR, which highly overexpresses MRP1.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.7% BSA, 0.1% sodium azide.
Specificity:
Recognizes a conformation-dependent internal epitope (aa617-932) of human MRP1 overexpressed in various human non-P-glycoprotein MDR tumor cell lines. Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, or mouse MRP gene products.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies: D.R. Hipfner, et al.; Cancer Res. 54, 5788 (1994) 2. Characterization of the M(r) 190,000 multidrug resistance protein (MRP) in drug-selected and transfected human tumor cell: K.C. Almquist, et al.; Cancer Res. 55, 102 (1995) 3. MRP and MDR1 gene expression in primary breast carcinomas: M. Filipits, et al.; Clin. Cancer Res. 2, 1231 (1996) 4. Multidrug resistance protein (MRP)-mediated transport of leukotriene C4 and chemotherapeutic agents in membrane vesicles. Demonstration of glutathione-dependent vincristine transport: D.W. Loe, et al.; J. Biol. Chem. 271, 9675 (1996) 5. MRP expression in acute myeloid leukemia. An update: M. Filipits, et al.; Adv. Exp. Med. Biol. 457, 141 (1999) 6. Monoclonal antibodies that inhibit the transport function of the 190-kDa multidrug resistance protein, MRP. Localization of their epitopes to the nucleotide-binding domains of the protein: D.R. Hipfner, et al.; J. Biol. Chem. 274, 15420 (1999) 7. Structural, mechanistic and clinical aspects of MRP1: D.R. Hipfner, et al.; Biochim. Biophys. Acta 1461, 359 (1999) 8. ATP- and glutathione-dependent transport of chemotherapeutic drugs by the multidrug resistance protein MRP1: J. Renes, et al.; Br. J. Pharmacol. 126, 681 (1999)