		Technical Data
M2825-13	MDR Protein (MRP1, Multidrug resistance-associated protein 1, ABCC1, ATP-binding cassette sub-family C member 1, Leukotriene C(4) transporter)

Description:
MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors .

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.2ug/ml (Anti mouse IgG labeled with HRP).
Immunocytochemistry: 1:20-1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations
Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate.
Flow Cytometry: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. (Note: Block and wash with solution containing 0.1% Tween.)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H406	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes human MDR protein with a defined linear epitope (aa918-924) of MRP1. Does not crossreact with human MDR1, MDR3, MRP2, MRP3, MRP4, MRP5 or MRP6 gene products. Does not crossreact with mouse mrp1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Cole, S.P.C. et al. (1992). Science 258: 1650-1654. 2. Hipfner, D.R. et al. (1999). Biochim. Biophys. Acta 1461: 359-376. 3. Hipfner, D.R. et al. (1994). Cancer Res. 54: 5788-5792. 4. Hipfner, D.R. et al. (1996). Cancer Res. 56: 3307-3314. 5. Wright, S. et al. (1998). Clin. Cancer Res. 4: 2279-2289.