Technical Data
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
P-glycoprotein, the product of the MDR1 (Multidrug Resistance 1) gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of the P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine, and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in the multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas, leukemias, and lymphomas

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.

Positive Control: Human liver or human colon
Expected Staining Pattern: Membrane
Epitope Retrieval: Not Required
Enzyme Digestion: Not Required

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ul-20CBlue IceMouse
Not determined
Multidrug resistant CHO cells
Purified from mouse ascites
Supplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.09% sodium azide
Recognizes Multidrug Resistance 1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.