Technical Data
M2825-14Q
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
P-glycoprotein, the product of the MDR1 (Multidrug Resistance 1) gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of the P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine, and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in the multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas, leukemias, and lymphomas

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.

Positive Control: Human liver or human colon
Expected Staining Pattern: Membrane
Epitope Retrieval: Not Required
Enzyme Digestion: Not Required

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6A122Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Multidrug resistant CHO cells
Purity:
Purified from mouse ascites
Form
Supplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide
Specificity:
Recognizes Multidrug Resistance 1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.