		Technical Data
M2825-14T	MDR1 (Multi Drug Resistance Protein 1, Multidrug Resistance Protein 1, MDR 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, MGC163296, P-glycoprotein 1, P-GP, PGY1)

Description:
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, and Immunohistochemstry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10 Fix cells in 10% Lysing solution.
Western Blot: 1:10.
Immunocytochemistry: 1:20 for acetone-fixed cells.
Immunohistochemstry: 1:20 for acetone-fixed frozen and formalin-fixed paraffin embedded tissues:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	JSB-1	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

MDR1

Purity:

Supernatant

Form

Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes a conserved cytoplasmic epitope of the plasma membrane-associated 170-180kD glycoprotein human MDR1. The expression is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. Species Crossreactivity: Chinese hamster. Does not crossreact with mouse or rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Scheper, R.J., et al., 1986, Int. J. Cancer 42, 389. 2. Dalton, W.S., et al., 1989, J. Clin. Oncol. 7, 415.
3. Tiirikainen, M., et al., 1992, Ann. Haematol. 65, 124. 4. Grogan, T., et al., 1993, Blood 81, 490.
5. Itsubo, M., et al., 1994, Cancer 73, 298. 6. Toth, K., et al., 1994, Am. J. Pathol. 144 227. 7. Gala, et al., 1994, J. Clin. Pathol. 47 619. 8. Sinicrope, et al., 1994, Cancer 74, 2908. 9. Baldini, N., et al., 1995, New Engl. J. Med. 333, 1380.