Technical Data
M2825-15F
MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)
Description:
CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.CD243 is expressed at low levels in the cell membrane of peripheral blood leukocytes, and constitutively
expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumor suppression and is therefore of special interest to oncologists. M2825-15F is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:200, Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9A183Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
Specificity:
Recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein). Species Crossreactivity: primate. Does not cross-react with mitochondrial pyruvate carboxylase, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828.
2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay. Cytometry Part A. 53A: 67-78.
3. Koziolek, M.J. et al. (2001) Expression of multidrug resistance P-glycoprotein in kidney allografts from
cyclosporine A-treated patients. Kid. Int. 60: 156-166.
4. Beck, W.T. et al. (1996) Methods to detect P-glycoprotein-associated multidrug resistance in patients’
tumours: Consensus recommendations. Cancer. Res. 56: 3010-3020.