Technical Data
M2825-18R
MDR3 P-glycoprotein (Multidrug Resistance Protein 3, ABC21, ATP-binding Cassette Sub-family B (MDR/TAP) Member 4, ABCB4, GBD1, PFIC-3, P-glycoprotein 3, PGY3)
Description:
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.
Immunohistochemistry: 1:20 on acetone fixed frozen sections. Does not work well on formaldehyde-fixed paraffin-embedded human tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2bP3II-26Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Fusion protein consisting of a fragment of MDR3 P-glycoprotein corresponding to aa629-692.
Purity:
Supernatant
Form
Supplied as a liquid in 0.7% BSA, 0.1% sodium azide.
Specificity:
Recognizes an internal epitope of MDR3 P-glycoprotein. Does not crossreact with human MDR1 P-glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scheffer, G.L., et al., Cancer Res. 60: 5269-5277 (2000). 2. Hooiveld, G.J., Gastroenterology 117: 678-687 (1999).