Technical Data
MDR3 P-glycoprotein (Multidrug Resistance Protein 3, ABC21, ATP-binding Cassette Sub-family B (MDR/TAP) Member 4, ABCB4, GBD1, PFIC-3, P-glycoprotein 3, PGY3)
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.

Suitable for use in Immunocytochemistry, Immunohistochemistry, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.
Immunohistochemistry: 1:20 on acetone fixed frozen sections. Does not work well on formaldehyde-fixed paraffin-embedded human tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceMouse
Not determined
Fusion protein consisting of a fragment of MDR3 P-glycoprotein corresponding to aa629-692.
Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.
Recognizes an internal epitope of MDR3 P-glycoprotein. Does not crossreact with human MDR1 P-glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scheffer, G.L., et al., Cancer Res. 60: 5269-5277 (2000). 2. Hooiveld, G.J., Gastroenterology 117: 678-687 (1999).