		Technical Data
M2867-60C	Mel18 (RNF110, MEL-18, Polycomb Group RING Finger Protein 2, DNA-binding Protein Mel-18, RING Finger Protein 110, Zinc Finger Protein 144, PCGF2, ZNF144)

Description:
The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Western Blot: 1-2ug/ml detects ~40kD band observed in Human Lung lysates (calculated MW of 37.8kD according to NP_009075.1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-KMTVNGAPVPPLT, from the C Terminus of human PCGF2 (NP_009075.1).

Purity:

Purified by peptide affinity chromatography.

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human PCGF2. Species sequence homology: canine, bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Xi S, Zhu H, Xu H, Schmidtmann A, Geiman TM, Muegge K. Lsh controls Hox gene silencing during development. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14366-71. 2. Elderkin S, Maertens GN, Endoh M, Mallery DL, Morrice N, Koseki H, Peters G, Brockdorff N, Hiom K. phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin.Mol Cell. 2007 Oct 12;28(1):107-20. 3. Endoh M, Endo TA, Endoh T, Fujimura Y, Ohara O, Toyoda T, Otte AP, Okano M, Brockdorff N, Vidal M, Koseki H.Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity. Development. 2008 Apr;135(8):1513-24.PMID: 183396754. Miki J, Fujimura Y, Koseki H, Kamijo T.Polycomb complexes regulate cellular senescence by repression of ARF in cooperation with E2F3.Genes Cells. 2007 Dec;12(12):1371-82.