Technical Data
MARCH4 (E3 Ubiquitin-protein Ligase MARCH4, Membrane-associated RING Finger Protein 4, Membrane-associated RING-CH Protein IV, MARCH-IV, RING Finger Protein 174, KIAA1399, RNF174)
RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa300-350 of human RNF174.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human MARCH4. Species Crossreactivity: chimpanzee, equine, mouse, rat. Species sequence homology: chicken, canine, opossum, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gerhard, DS. et al. Genome. Res. 14:2121-2127 (2004). 2. Bartee, E. et al. J. Virol. 78:1109-1120 (2004).