Technical Data
c-Met (NT) (Hepatocyte Growth Factor, Scatter Factor Receptor, HGF, SF, Hepatocyte Growth Factor Receptor Precursor, HGF Receptor, HGFR, HGF/SF Receptor, Met Proto-oncogene Tyrosine Kinase, Oncogene MET, Renal Cell Carcinoma Papillary 2 Gene, RCCP2, SF Re
Met is a receptor protein kinase (RPTK) for a multifunctional cytokine called (HFG) which controls cell growth and motility. Met has distinctive features that distinguish it from other members of RPTK's.

Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000. 293 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with M3007-03F (1:2000). Proteins were visualized using a IgG (HRP) Goat anti-rabbit and a chemiluminescence detection system.
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (Paraffin): 1:100-1:250
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt

Positive Control:
293 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to residues near the N-terminus of human Met. Cellular Localization: Membrane; Single-pass type I membrane protein.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide.
Recognizes human Met at and around the N-terminus at ~190kD (full length) and 56kD (cleaved).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, D, et al. (2002). J Biol Chem 277(39):36216-36222. 2. Chan, A., et al. (1987). Oncogene 1(2):229-233. 3. Schmidt, L.., et al. (1997). Nat Genet. 16(1):68-73