Technical Data
M3007-03F
c-Met (NT) (Hepatocyte Growth Factor, Scatter Factor Receptor, HGF, SF, Hepatocyte Growth Factor Receptor Precursor, HGF Receptor, HGFR, HGF/SF Receptor, Met Proto-oncogene Tyrosine Kinase, Oncogene MET, Renal Cell Carcinoma Papillary 2 Gene, RCCP2, SF Re
Description:
Met is a receptor protein kinase (RPTK) for a multifunctional cytokine called (HFG) which controls cell growth and motility. Met has distinctive features that distinguish it from other members of RPTK's.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000. 293 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with M3007-03F (1:2000). Proteins were visualized using a IgG (HRP) Goat anti-rabbit and a chemiluminescence detection system.
Immunocytochemistry: 1:100-1:250
Immunohistochemistry (Paraffin): 1:100-1:250
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt

Positive Control:
293 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i174Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues near the N-terminus of human Met. Cellular Localization: Membrane; Single-pass type I membrane protein.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide.
Specificity:
Recognizes human Met at and around the N-terminus at ~190kD (full length) and 56kD (cleaved).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, D, et al. (2002). J Biol Chem 277(39):36216-36222. 2. Chan, A., et al. (1987). Oncogene 1(2):229-233. 3. Schmidt, L.., et al. (1997). Nat Genet. 16(1):68-73