Technical Data
M3007-13
c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Description:
Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor. The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain. Both chains are derived from a single precursor protein by proteolytic cleavage. c-Met is expressed in a wide variety of adult and embryonic tissues. It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF. Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas. c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis. Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1ug/ml
Western Blot: 1-2ug/ml
Immunohistochemistry (Paraffin): 1-2ug/ml. Best results obtained by subjecting tissue to heat-induced epitope retrieval (HIER).
Optimal dilutions to be determined by the researcher.

Positive Controls:
Western Blot: A431 cell extracts
Immunohistochemistry: Human breast cancer tissue

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Available without azide, BSA and glycerol. See M3007-13A.
Specificity:
Recognizes the human c-Met protein at ~145kD. Does not crossreact with related proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Seol DW, et al. 1999. Biol. Chem. 274:3565-3572