Technical Data
M3885-48F
MHC Class 1 (FITC)
Description:
Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.

Applications:
Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50 (80ul/test) Antibody (whole molecule) is cytotoxic in the presence of complement.
Optimal dilutions to be determined by the researcher.

Flourescein/IgG molar ratio: 7.4

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7H121Affinity Purified
SizeStorageShippingSourceHost
120 Tests4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
Human HSB-2 T cell line.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM sodium chloride 5% Glycerol, 0.2% BSA, 0.04% sodium azide.
Specificity:
Recognizes a monomorphic Class I epitope on the a chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. G.S. Eisenbarth, et al, (1980) J Immunol 124: 1237-1244. 2. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. 3. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194.
4. K.L. Rock, (1996) Immunol Today 17: 131-137. 5. J Immunol (1985) 135(3): 1752-1759. 6.. A. Kawana-Tachikawa, A. Iwamoto, et al (2002) J Virol 76(23):11982-11988. 7. J.E. Shaw, R.W. Lang, et al (2001) J Virol Meth 94: 137-146.