Description:
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatability 2 (H-2) region.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
X63 myeloma cells with spleen cells from C1D mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 10F80 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Mouse | Mouse |
|
| Concentration: |
| ~0.1mg/ml |
| Immunogen: |
| Purified H-2Kb and H-2Db MHC-I molecules. |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC). |
| Specificity: |
| Recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognise a polymorphic epitope of canine MHC class 1. Species Crossreactivity: rat, guinea pig, sheep, bovine, porcine, human and hamster. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Claesson, M.H. et al. (1994) Antibodies directed against monomorphic and evolutionary conserved self epitopes may be generated in 'knock-out' mice. Development of monoclonal antibodies directed against monomorphic MHC class I determinants. Scand. J. Immunol. 40: 257 - 264. 2.Vitadello M et al., Arthritis Res Ther 12: R52 (2020)
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