Technical Data
M3885-48Q3
MHC Class 1 (MHC Class I) (FITC)
Description:
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatability 2 (H-2) region.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63 myeloma cells with spleen cells from C1D mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2b10F80Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified H-2Kb and H-2Db MHC-I molecules.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognise a polymorphic epitope of canine MHC class 1. Species Crossreactivity: rat, guinea pig, sheep, bovine, porcine, human and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Claesson, M.H. et al. (1994) Antibodies directed against monomorphic and evolutionary conserved self epitopes may be generated in 'knock-out' mice. Development of monoclonal antibodies directed against monomorphic MHC class I determinants. Scand. J. Immunol. 40: 257 - 264. 2.Vitadello M et al., Arthritis Res Ther 12: R52 (2020)