Technical Data
M3885-59B
MHC Class 1, H2, Db, alpha3 (FITC)
Description:
MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin.3, 4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3, 6 This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin.7,8

Applications:
Flow cytometry
Immunohistochemistry (frozen tissue sections)
Complement-mediated cytotoxicity

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k28-14-8Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
C3H.SW mouse splenocytes
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with FITC.
Specificity:
Recognizes an epitope in the alpha3 domain of H-2Db.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. J. Immunol. 125:2473.
2. Ozato, K., and D.H. Sachs. 1981. J. Immunol. 126:317.
3. Lawlor, D.A., et al. 1990. Annu. Rev. Immunol. 8:23.
4. Bjorkman, P.J., et al. 1987. Nature 329:506.
5. Yewdell, J.W., and J.R. Bennett. 1990. Cell 62:203.
6. Zijlstra, M., et al. 1990. Nature 344:742.
7. Allen, H., D. Wraith, P. Pala, B. Askonas, and R.A. Flavell. 1984. Nature 309:279.
8. Allen, H, J. Fraser, D. Flyer, S. Calvin, and R. Flavell. 1986. Proc. Natl. Acad. Sci. USA 83:7447
9. Woodward, J.G., A. Orn, R.C. Harmon, R.S. Goodenow, L. Hood, and J.A. Frelinger. 1982. Proc. Natl. Acad. Sci. USA 79:3613.
10. Orn, A., R.S. Goodenow, L. Hood, P.R. Brayton, J.G. Woodward, R.C. Harmon, and J.A. Frelinger. 1982. Nature 297:415.
11. Kundig, T.M., M.F. Bachmann, C. DiPaolo, et al. 1995. Science 268:1343.
12. Mason, L.H., J.R. Ortaldo, H.A. Young, V. Kumar, M. Bennett, and S.K. Anderson. 1995. J. Exp. Med. 182:293.