Technical Data
MHC Class 1, H2, Db, alpha3 (FITC)
MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.

Suitable for use in Flow Cytometry and Immunohistochemistry (Frozen). Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ug-20CBlue IceMouseMouse
C3H.SW mouse splenocytes
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Recognizes the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin. Crossreacts with the alpha3 domain of H-2Ld. Does not react with Kd, Dd, H-2Dq, and/or Lq. Blocks H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ozato, K., et al., J. Immunol. 125: 2473-2477 (1980). 2. Allen, H., et al., Proc. Natl. Acad. Sci. USA 83: 7447-7451 (1986). 3. Lie, W., et al., J. Exp. Med. 173: 449-459 (1991). 4. Levitsky, H.I., et al., J. Exp. Med. 179: 1215-1224 (1994). 5. Ozato, K. & Sachs, D.H., J. Immunol. 126: 317-321 (1981). 6. Woodward, J.G., et al., Proc. Natl. Acad. Sci. USA 79: 3613-3617 (1982). 7. Wang, R., et al., 157: 2961-2968 (1996). 8. Lee, S., et al., Cancer Gene Ther. 11: 237-248 (2004).