Technical Data
MHC Class 1, H2, Db, alpha3 (Biotin)
Flow cytometry1,2
Immunohistochemistry (acetone-fixed, frozen tissue sections only)
Complement-dependent cytoxicity assay1,2

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ug-20CBlue IceMouseMouse
C3H.SW splenocytes
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Recognizes the alpha3 domain of H-2Db class I MHC antigen. Crossreacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ozato, K., T.H. Hansen, and D.H. Sachs. 1980. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125:2473-2477.
2. Ozato, K., and D.H. Sachs. 1981. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126:317-321.