Technical Data
M3886-12X
MHC Class 1 HLA ABC (BSA and Azide Free)
Description:
Human MHC class I antigens (HLA A, B and C) are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections only. Acetone fixation is receommended.
The optimal dilution should be determined by the researcher..

Positive Controls:
Human lymphocytes. Tonsil and lymph node

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3F273Affinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Cell membranes of human tonsil lymphocytes. Cellular Localization: Cell membrane.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See M3886-12.
Specificity:
Recognizes a monomorphic determinant of human major histocompatibility (MHC) class I antigens.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Appleyard S T, et al. Lancet. I: 361–3 (1985). 2. Daar A S, et al. Transplantation. 38:287–92 (1984). 3. Muller C, et al. Eur. J. Immunol. 13: 414–8 (1938).