Technical Data
Media
Middlebrook 7H11 Mycobacteria Agar (Powder)
M3895-06
Microbiological Grade
Storage RT/4°C    Shipping RT
Formulation per Liter g/Liter
Components
Agar13.5
Sodium Phosphate, Dibasic1.5
Potassium Phosphate, Monobasic1.5
Pancreatic Digest of Casein1.0
Ammonium Sulfate0.5
Monosodium Glutamate0.5
Sodium Citrate0.4
Ferric Ammonium Citrate0.04
Magnesium Sulfate0.05
Copper Sulfate0.001
Pyridoxine0.001
Zinc Sulfate0.001
Biotin0.0005
Calcium Chloride0.0005
Malachite Green0.00025
Total:19g/Liter
Used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H10 or 7H9 formulations.


Directions per Liter: Mix 19g of medium into 900ml of distilled/deionized (DDI) water and 5ml glycerol. Heat to boiling until dissolved completely. Autoclave for 10 minutes at 121ºC (15psi). Cool to 45-50°C and aseptically add 100ml of OADC enrichment.

Storage and Stability: Store powdered media at RT or 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light blue-beige, homogeneous, free- flowing powder

Solubility: Grey-white to pale light green, trace to slightly hazy

pH (25°C): 6.6 ± 0.2

Expected Culture Response: 3-28 days at 35°C under CO2.

Growth Analysis: Mycobacterium kansasii Group I ATCC 12478 Mycobacterium scrofulaceum Group II ATCC 19981 Mycobacterium intracellulare Group III ATCC 13950 Mycobacterium fortuitum Group IV ATCC 6841 Mycobacterium tuberculosis H37Ra ATCC 25177


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. APJH, 98:844 (1968) 2. Am. Rev. Resp. Dis., 98:925 (1968).