Technical Data
Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)
Molecular Biology Storage: -20CShipping: Blue Ice
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector. It consists of 151 amino acids.

Molecular Weight: 16.6kD (151 amino acids)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, Recombinant, E. coli
Purity: 95% by SDS-PAGE. Purified by conventional column chromatography.
Concentration: ~1mg/ml
Form: Supplied as a colorless, clear solution in PBS, pH 7.4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Weiser, WY., et al., Proc. Natl. Acad. Sci. 86: 7522-7526 (1989). 2. Bernhagen, J., et al., Biochemistry 33: 14,144-14,155 (1994). 3. Richard, B., FASEB J. 10: 1607-1613 (1996).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.