Technical Data
M3895-07J
Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)
100ug
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Technical Data |
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M3895-07J |
Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector. It consists of 151 amino acids. Molecular Weight: 16.6kD (151 amino acids) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 16.6kD |
Source: Human, Recombinant, E. coli Purity: 95% by SDS-PAGE. Purified by conventional column chromatography. Concentration: ~1mg/ml Form: Supplied as a colorless, clear solution in PBS, pH 7.4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Weiser, WY., et al., Proc. Natl. Acad. Sci. 86: 7522-7526 (1989). 2. Bernhagen, J., et al., Biochemistry 33: 14,144-14,155 (1994). 3. Richard, B., FASEB J. 10: 1607-1613 (1996).
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