Technical Data
M3895-07W
MIF, Recombinant, Human (Macrophage Migration Inhibitory Factor, Glycosylation-inhibiting Factor, GIF, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase, GLIF, MMI)
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
MIF is involved in cell-mediated immunity, immunoregulation, and inflammation. Reportedly, it also plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signalling pathways. However, the biological mechanism and significance of MIF remains unknown.

Source:
Recombinant corresponding to aa101-445 from mature human MIF, fused to 6x His-tag at N-terminal, expressed in E. coli.

Applications:
Suitable for use in ELISA, Western Blot, Dot Blot, Immunohistohemistry, Immunoassays, Development of Rapid tests and other immunoassay. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: As reported
Form: Supplied as liquid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Weiser W.Y. et al (1989). Proc. Natl. Acad. Sci. U.S.A. 86 (19) 7522-6. 2. Kozak C.A. et al (1995). Genomics 27 (3) 405-11.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.