Technical Data
Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)
Molecular Biology Storage: -20°CShipping: Blue Ice
Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogensis and has several important biological effects, including promotion of neurite extension and neuronal survival. Recombinant Midkine is a 13.4kD protein, comprising of 123 amino acid residues.

Biological Activity:
Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10ng/ml.

AA Sequence:

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile dH2O, and a carrier protein. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
Form: Supplied as a sterile filtered, lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O, carrier protein to 0.1-1mg/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. [Expression of midkine in patients with acute myeloid leukemia and its significance.]
Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006 Jun;14(3):442-5
2. Two-step transcriptional amplification-lipid-based nanoparticles using PSMA or midkine promoter for suicide gene therapy in prostate cancer.
Cancer Sci 2006 Jun 23;
3. [Expression of midkine in eutopic and ectopic endometrium of patients with severe endometriosis and its clinical significance.]
Zhonghua Fu Chan Ke Za Zhi 2006 May;41(5):299-301
4. Midkine is a potent regulator of the catecholamine biosynthesis pathway in mouse aorta.
Life Sci 2006 Mar 21;
5. Mice doubly deficient in the midkine and pleiotrophin genes exhibit deficits in the expression of beta-tectorin gene and in auditory response.
Lab Invest 2006 Jul;86(7):645-53
6. Doxorubicin-conjugated anti-midkine monoclonal antibody as a potential anti-tumor drug.
Jpn J Clin Oncol 2006 Apr;36(4):207-11

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.