Technical Data
Media
Minimal Sporulation Medium (Powder)
M3900
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Potassium Acetate10g
Agar20g
500g makes 16.7 liters
Used to sporulate MATa/MAT diploid cells after 18-24 hours. Nutritional supplements are required for sporulation of auxotrophic diploids.


Directions per Liter: Dissolve 30 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White to light tan, homogeneous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Kaiser, C., et al., Methods in Yeast Genetics, Cold Spring Harbor Laboratory Press, Appendix A: 213 (1994)