Technical Data
M3949A
MIG, Recombinant, Human (Monokine induced by Interferon Gamma)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to human MIG expressed in E. coli.

Molecular Weight:
~12kD

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 range=10.0-100ng/ml, determined by the dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2. The optimal concentration for each specific application should be determined by an initial dose response assay.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~12kD
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Farber, J.M. (1993) HuMig: a new human member of the chemokine family of cytokines. Biochem. Biophys. Res. Commun. 192:223-230. 2. Liao, F., R.L. Rabin, J.R. Yanelli, L.G. Koniaris, P. Vanguri, and J.M. Farber (1995) Human Mig chemokine: biochemical and functional characterization. J. Exp. Med. 182:1301-1314. 3. Loetscher, M., B. Gerber, P. Loetscher, S.A. Jones, L. Piali, I. Clark-Lewis, M. Baggiolini, and B. Moser (1996) Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J. Exp. Med. 184:963-969. 4. Weng, Y., S.J. Siciliano, K.E. Waldburger, A. Sirotina-Meisher, M.J. Staruch, B.L. Daugherty, S.L. Gould, M.S. Springer, and J.A. DeMartino (1998) Binding and functional properties of recombinant and endogenous CXCR3 chemokine receptors. J. Biol. Chem. 273:18288-18291.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.