Technical Data
M3949A
MIG, Recombinant, Human (Monokine induced by Interferon Gamma)
10ug
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Technical Data |
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M3949A |
MIG, Recombinant, Human (Monokine induced by Interferon Gamma) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to human MIG expressed in E. coli. Molecular Weight: ~12kD Endotoxin: ~0.1ng/ug Biological Activity: ED50 range=10.0-100ng/ml, determined by the dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2. The optimal concentration for each specific application should be determined by an initial dose response assay. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~12kD |
Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Farber, J.M. (1993) HuMig: a new human member of the chemokine family of cytokines. Biochem. Biophys. Res. Commun. 192:223-230. 2. Liao, F., R.L. Rabin, J.R. Yanelli, L.G. Koniaris, P. Vanguri, and J.M. Farber (1995) Human Mig chemokine: biochemical and functional characterization. J. Exp. Med. 182:1301-1314. 3. Loetscher, M., B. Gerber, P. Loetscher, S.A. Jones, L. Piali, I. Clark-Lewis, M. Baggiolini, and B. Moser (1996) Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J. Exp. Med. 184:963-969. 4. Weng, Y., S.J. Siciliano, K.E. Waldburger, A. Sirotina-Meisher, M.J. Staruch, B.L. Daugherty, S.L. Gould, M.S. Springer, and J.A. DeMartino (1998) Binding and functional properties of recombinant and endogenous CXCR3 chemokine receptors. J. Biol. Chem. 273:18288-18291.
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