Technical Data
M3985-06
Mitochondria
Description:
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.

Applications:
Suitable for use in Indirect Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Can also be used for isolation of intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads. Other applications have not been tested.

Recommended Dilution:
Indirect Immunofluorescence: 1:10-1:50, 30-100ul per slide well
Immunohistochemistry (paraffin-embedded tissues): 1:80 Using heart ventricle epithelium pretreated with EDTA, pH 8.0. Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells. Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Immunohistochemistry: 1:100. Works best with organic fixatives (acetone, methanol, etc.). 2% PFA for 10-15 minutes. can be used but stronger use of formalin or 4% PFA limits staining.
Optimal dilutions to be determined by the researcher.

Positive Control: COS7 cells, heart ventricle

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2230Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human mitochondria.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS pH 7.2. No preservatives.
Specificity:
Recognizes human mitochondria. Gives mitochondrial staining on all human cell types. This pattern is best described as a spaghetti- like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells. Recognizes a 65kD protein by immunoprecipitation. Does not cross-react with rat and mouse. Epitope: surface of intact mitochondria.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cell Regulation (1991) 2:1097–1113.2. J. of Neuroscience. (1998) 18(19):7674–7686. 3. Reubinoff, B.E., et al., Nature Biotechnology (2001) 19:1134–1140.