Technical Data
M3985X
Mitochondria (BSA & Azide Free)
Description:
This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations. Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells

Applications:
Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 minutes at RT.
Note: Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells and liver

Cellular Localization:
Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.424Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Semi-purified mitochondrial preparation.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added. No stabilizing protein added. Also supplied with BSA and sodium azide. Refer to M3985.
Specificity:
Recognizes a human 60kD non-glycosylated protein component of mitochondria found in human cells. Epitope: Not determined. Species crossreactivity: Human. Others not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.