Technical Data
M3985X
Mitochondria (BSA & Azide Free)
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Technical Data |
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M3985X |
Mitochondria (BSA & Azide Free) |
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Description: This monoclonal antibody is part of a new panel of reagents which recognize subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations. Murine anti-mitochondria monoclonal antibody produces a "spaghetti"-like staining pattern in the cytoplasm of normal and malignant human cells Applications: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells and liver Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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